Structural Analysis of Recombinant AAV Vector Genomes at Single-Molecule Resolution
July 31, 2026
AAV vector preparations can contain a range of genome structures that may impact vector quality and manufacturing. This paper explores a long-read sequencing approach that enables individual AAV genomes to be characterised at single-molecule resolution, providing deeper insight into genome heterogeneity and the factors that may contribute to it.
What you’ll learn:
- How individual AAV genome structures can be identified and classified
- Where structural variants occur within the vector genome
- What sequence and structural features may contribute to genome heterogeneity
- How these insights can inform AAV vector design and manufacturing
Why it matters:
Deeper insight into AAV genome structure can help teams better understand vector quality, strengthen product characterisation, and identify opportunities to optimise vector design and manufacturing.
Read the full paper to explore the approach and findings.
